Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10235448 | Process Biochemistry | 2014 | 6 Pages |
Abstract
A microbial biosensing system for detection of hydrogen sulfide has been developed by using immobilized Thiobacillus thioparus TK-m in poly vinyl alcohol matrix, together with a dissolved oxygen sensor. Parameters of immobilization (poly vinyl alcohol concentration and amount of wet cell) were optimized by using statistical software. The obtained values for concentration of poly vinyl alcohol and wet cell weight were 11.3% (w/v) and 45Â mg, respectively, where the response time of biosensor was 80Â s. Calibration of oxygen concentration based on hydrogen sulfide concentration was investigated between 1Â mg/L and 20Â mg/L. The effect of pH and temperature were investigated in specific range of experimental conditions as well. Some parameters including operational stability and detection limit were studied in detail for characterization of biosensing system. In order to determine the operational stability, bio-sensing system at optimized working conditions was used to distinguish viability of microorganisms in polymer beads in period of time.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Maryam Mirzaei, Ghassem Amoabediny, Fatemeh Yazdian, Mojgan Sheikhpour, Ehsan Ebrahimi, Bahman Ebrahimi Hossein Zadeh,