| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10235516 | Process Biochemistry | 2012 | 5 Pages |
Abstract
⺠A fully active easily-labeled INSL3 analog was designed and recombinantly prepared. ⺠The analog was easily mono-labeled by DTPA/Eu3+-moiety at the A-chain N-terminus. ⺠The europium-labeled INSL3 retained high binding affinity with receptor RXFP2. ⺠A convenient RXFP2-binding assay was established using the non-radioactive tracer. ⺠The high throughput assay will facilitate screening of novel agonists or antagonists.
Keywords
DTPAIBMXTRFDOTATFAINSL31,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid3-isobutyl-1-methylxanthineBSADMSOReceptor-binding assaybovine serum albuminTrifluoroacetic acidDiethylenetriaminepentaacetic acidcircular dichroismultra-violetTime-resolved fluorescenceinsulin-like peptide 3Insulin-like peptidehigh performance liquid chromatographyHPLC
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Wei-Jie Zhang, Xue-Juan Gao, Ya-Li Liu, Xiao-Xia Shao, Zhan-Yun Guo,