A thermostable variant of P. aeruginosa cold-adapted LipC obtained by rational design and saturation mutagenesis

► LipC is a cold-acting lipase showing a relatively low thermal resistance. ► A rational design system was developed to modify specific sites on the enzyme structure. ► A variant showing a 7-fold increase in thermo resistance could be successfully generated. ► A stable, cold adapted LipC bearing higher thermal stability was obtained. ► This is the first time a cold adapted enzyme is converted into a more thermo-resistant variant.