| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10235555 | Process Biochemistry | 2012 | 7 Pages |
Abstract
⺠We have isolated three toluene-tolerant Escherichia coli mutant strains via engineering its global transcription factor cAMP receptor protein (CRP). ⺠The mutant isolation was achieved within 3-5 days through directed evolution of CRP. ⺠The best mutant M2 was also tolerant against other organic solvents such as n-hexane, cyclohexane and p-xylene. ⺠qRT-PCR proved the differential expression of certain organic-solvent stress related genes in the mutant.
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Authors
Souvik Basak, Hao Song, Rongrong Jiang,