| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10235614 | Process Biochemistry | 2013 | 8 Pages |
Abstract
⺠A cell-based fluorescence assay was developed for screening cytotoxic effects of drugs and TCHMs. ⺠Mouse embryonic stem and human colon and breast cancer cells expressing EGFP were cultured in 3-D. ⺠Drug effects on cell proliferation or death were monitored via the dynamic culture fluorescence. ⺠The results from 3 TCHMs validated the 3-D fluorescent culture screening method. ⺠The 3-D fluorescent assay is more sensitive and reliable than 2-D and cell counting assay.
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Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Ding Li, Ru Zang, Shang-Tian Yang, Jufang Wang, Xiaoning Wang,