| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10235666 | Process Biochemistry | 2012 | 7 Pages |
Abstract
⺠Fab produced in Escherichia coli medium cell densities chemostats at 37, 33 and 30 °C. ⺠Product yield increased as temperature decreased within working range. ⺠Biologically active 3H6 Fab produced at the three temperatures. ⺠30% of total Fab secreted to periplasm, irrespective of process temperature. ⺠Considerable product leakage to the culture supernatant detected in all cases.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Escarlata RodrÃguez-Carmona, Olivia Cano-Garrido, Martin Dragosits, Michael Maurer, Alexander Mader, Renate Kunert, Diethard Mattanovich, Antonio Villaverde, FelÃcitas Vázquez,