| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10235735 | Process Biochemistry | 2011 | 6 Pages |
Abstract
⺠UDP-glucose 6-dehydrogenase was cloned and characterizated. ⺠A full-length cDNA (1846 bp) encoding UDP-GlcDHase was obtained by PCR using degenerate primers and the RACE strategy. ⺠The cDNA encoded a protein of 520 amino acids and about 50% were high degree of conservation in the nucleotide binding and catalytic domains. ⺠The cDNA was cloned in the Pichia pastoris GS115 (pPIC3.5k) and recombinant UDP-GlcDHase enzymatic assay was characterized. ⺠The biochemical properties of the purified recombinant enzyme were comparable with those of its homologs.
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Authors
Tian Zhou, Yong C. Song, Ming Q. Feng, Ren X. Tan,