| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10235753 | Process Biochemistry | 2011 | 6 Pages |
Abstract
⺠We have described the kinetic behavior of alcohol (ADH) and aldehyde (AldDH) dehydrogenases in solution and immobilized onto carbon platform via polyamidoamine (PAMAM) dendrimers. ⺠The influence of pH, K+, thiol and temperature was evaluated. ⺠The results showed that PAMAM dendrimers provide a good environment for the immobilization of dehydrogenase enzymes. ⺠The employed anchoring methodology (layer-by-layer) required very low catalyst consumption, and the anchored enzymes were quite stable over a period of approximately 30 days.
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Authors
Sidney Aquino Neto, Juliane C. Forti, Valtencir Zucolotto, Pietro Ciancaglini, Adalgisa R. De Andrade,