Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10235785 | Process Biochemistry | 2013 | 7 Pages |
Abstract
The α,β-unsaturated carbonyl compound (4S)-(+)-carvone was selectively reduced to (1R,2R,4S)-iso-dihydrocarveol using baker's yeasts. The conversion of the bioreduction reaction was monitored using a green hollow-fiber liquid-liquid-liquid microextraction (HF-LLLME) technique. Several parameters which may affect the bioreduction of (4S)-(+)-carvone, such as temperature, time, substrate/enzyme ratio, pH and buffer concentration, were evaluated. The effect of some additives, such as trehalose, DMSO and the ionic liquid [BMIm][PF6], was also studied. The (1R,2R,4S)-iso-dihydrocarveol was recovered with 52.7% conversion and diastereoisomeric excess >99% after 48 h of reaction at 40 °C in an aqueous monophasic system, with 0.1 mol Lâ1 buffer concentration (pH 7.5) and a substrate/yeast cell mass ratio of 8.0 mg gâ1. The HF-LLLME microextraction technique allowed the optimization of the reaction with a reduction of over 99.5% in relation to the use of organic solvents.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Vanessa Dutra Silva, Jeferson Schneider Carletto, Eduardo Carasek, Boris Ugarte Stambuk, Maria da Graça Nascimento,