| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10235892 | Process Biochemistry | 2011 | 8 Pages |
Abstract
⺠Monomeric chains c, from the trimer abc reduction, have four isoforms with MM in the range 17,336-17,620 Da. ⺠Two isoforms, T1 and T2, with MM 51,200 and 51985 Da, are observed for the trimer abc subunit. ⺠Based on SDS-PAGE, the linker chains L have a high affinity for the trimer abc, appearing on fractions I-V. ⺠The strong interaction between the linkers and trimer abc makes it difficult the purification of these subunits. ⺠Monomer d fraction VI appears to be very pure, in agreement with previous studies.
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Authors
Francisco Adriano O. Carvalho, José Wilson P. Carvalho, PatrÃcia S. Santiago, Marcel Tabak,