Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10236178 | Process Biochemistry | 2005 | 4 Pages |
Abstract
The conversion of cholesterol (C) to androstenedione (AD) and androstadienedione (ADD) was influenced significantly by the composition of the cultivation medium. A medium composed of (g/l): glucose, 50; peptone, 20; corn steep solid, 3; supported relatively high AD and ADD outputs. C convertibility was further accelerated when the cultivation medium was adjusted with phosphate buffer with the pH range 5.5-7.38. Supplementation of the medium with 8-hydroxyquinoline or methanol prevented the degradation of the C nucleus with concomitant formation of good AD and ADD yields.
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Authors
Lotfy A.R. Sallam, Abdel-Monem El-Refai, Hassan A. El-Minofi,