Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10236277 | Process Biochemistry | 2005 | 5 Pages |
Abstract
A novel ethylenediaminetetraacetate (EDTA)-degrading bacterial strain (LPM-4) with a specific requirement for EDTA as the sole nitrogen source was isolated. According to 16S rDNA sequencing, this isolate belonged to the α-proteobacteria group and was closely related to uncultured bacteria available from the GenBank (NCBI) database. The cells consumed glucose added to EDTA-containing medium only after EDTA exhaustion. Mass cell yield (Yx/s) from EDTA and glucose amounted to 22.4 and 25.5%, respectively; the energy cell yield (ηx/s) from both substrates was 32%. Suspensions of washed cells of this isolate degraded EDTA and complexes Baâ, Mgâ, Caâ, and MnâEDTA at constant rates ranging from 0.310 to 0.486 mmol EDTA/(g dry cells h). Specific degradation rate of the more stable complex ZnâEDTA was 0.137±0.016 mmol EDTA/(g dry cells h) within the first 10 h of incubation and then declined.
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Authors
T.I. Chistyakova, E.G. Dedyukhina, A.D. Satroutdinov, E.N. Kaparullina, E.Yu. Gavrish, V.K. Eroshin,