Correlation between key enzyme activities in the inosine synthetic pathway and inosine production

The specific activities of key enzymes relating to the pathway of inosine synthesis of three different bacterial strains including high-yield, low-yield and wild strains were determined and compared systematically. A close relationship between inosine production and the specific activities of key enzymes was found. According to the enzyme characteristics of the high-yield strain, suggestions on further strain improvement by genetic engineering were proposed. An enzymology study is suggested as an effective way to facilitate the screening of high-yield strain.