Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10236378 | Process Biochemistry | 2005 | 6 Pages |
Abstract
Genetically engineered Escherichia coli JM109, which expressed merT-merP protein and metallothionein (MT), was employed in this study to evaluate its potential for mercury bioaccumulation accompanied by simultaneous cell propagation in Hg2+ solution containing organic matters. In contrast to original host, E. coli JM109 which could hardly grow at 1Â mg/L Hg2+ concentration, genetically engineered E. coli cells were able to propagate themselves in LB with Hg2+ concentration up to 7.4Â mg/L, although cell reproduction was delayed with increasing Hg2+ concentration. Simultaneously, this strain could accumulate Hg2+ from LB + Hg2+ solutions effectively with more than 96% of mercury removal whether the cells were induced by IPTG or not. Cell propagation was somewhat inhibited by IPTG, but the capability of Hg2+-uptake of the induced cells was enhanced. These results suggested this strain could be used for the bioremediation of contaminated wastes containing mercury and organic matters.
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Authors
X.W. Zhao, M.H. Zhou, Q.B. Li, Y.H. Lu, N. He, D.H. Sun, X. Deng,