Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10267032 | Electrochemistry Communications | 2005 | 4 Pages |
Abstract
A strategy for the preparation of tyrosinase biosensor has been proposed to determine dopamine metabolite of 3,4-dihydroxyphenylacetic acid (DOPAC). An amino-polysaccharide chitosan was successfully used as matrix to entrap tyrosinase on glassy carbon electrode surface on basis of tyrosinase conjugated with chitosan. The sensor was operated at â0.15Â V. The current linearly increased with the increasing concentration of DOPAC over the concentration of 6Â nM to 0.2Â mM. The sensor can be used to selectively determine nanomolar DOPAC in the presence of physiological level of ascorbic acid (AA), uric acid (UA), acetaminophen, neurotransmitters including dopamine, l-dopa, adrenaline and noradrenaline.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Chemical Engineering (General)
Authors
Aihua Liu, Itaru Honma, Haoshen Zhou,