Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10410346 | Sensors and Actuators B: Chemical | 2005 | 7 Pages |
Abstract
This paper reports the detection of Escherichia coli (E. coli) O157:H7 with a surface plasmon resonance (SPR) sensor using three sample preparation methods: untreated (viable), heat-killed then soaked in 70% ethanol, and detergent-lysed. The SPR sensing surface consists of a monoclonal antibody immobilized onto a mixed -COOH and -OH terminated self-assembled monolayer (SAM) of alkanethiols on a gold surface. The limit of detection (LOD) for each method is determined by the minimum measurable shift in resonant wavelength corresponding to the specific binding of E. coli O157:H7 and the subsequent binding of an antibody for amplification. Detergent-lysed samples produce the lowest LOD at 104Â cfu/ml, while the LOD is 105Â cfu/ml for heat-killed samples and 106Â cfu/ml for untreated samples, respectively. Possible reasons for different limits of detection are discussed.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Allen D. Taylor, Qiuming Yu, Shengfu Chen, JiÅÃ Homola, Shaoyi Jiang,