Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10410431 | Sensors and Actuators B: Chemical | 2005 | 5 Pages |
Abstract
A new film of Triton X-100 to incorporate hemoglobin for biosensor fabrication is presented in this paper. In this film, hemoglobin displays a pair of redox peaks in pH 6.0 NaAc-HAc buffer solution with a formal potential of â0.257Â V (versus SCE) and shows a thin-layer behavior. Without electron mediator or promoter, the modified electrode has an electrocatalytic activity to the reduction of hydrogen peroxide (H2O2), which shows a linear dependence on the H2O2 concentration ranging from 1.0 Ã 10â6 to 1.0 Ã 10â4Â mol/L. The detection limit of 3.0 Ã 10â7Â mol/L is estimated when the signal-to-noise ratio is 3. The KMapp value of hemoglobin in Triton X-100 film has been determined to be 4.27Â mmol/L. Ascorbate, uric acid, dopamine, catechol, cystine and epinephrine will not interfere with the sensitive determination of H2O2.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xinjian Liu, Yi Xu, Xiang Ma, Genxi Li,