Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10410449 | Sensors and Actuators B: Chemical | 2005 | 7 Pages |
Abstract
Horseradish peroxidase (HRP) to a nano-Au monolayer, which was supported by PAMAM dendrimer/cystamine modified gold electrode. The high affinity of PAMAM dendrimer for nano-Au with its amine groups was used to realize the role of nano-Au as an intermediator to retain the high bioactivity of HRP. A hydrogen peroxide biosensor was fabricated based on the effective immobilization of the high bioactivity of HRP. In the presence of the hydroquinone mediator in the solution, the immobilized HRP exhibited excellent electrocatalytical response to the reduction of H2O2. The effects of various experimental variables were optimized. The resulting biosensor showed a linear response to H2O2 over a concentration range from 1 à 10â5 to 2.5 à 10â3 mol Lâ1 with a sensitivity of 0.53 A L molâ1 cmâ2 and a detection limit of 2.0 μmol Lâ1 based on a signal-to-noise ratio of 3. The apparent Michaelis-Menten constant (Kmapp) of the biosensor was evaluated to be 0.52 mmol Lâ1. The biosensor exhibited high sensitivity and good stability and reproducibility. Performance of the biosensor was evaluated with respect to possible interferences and the application to real sample analysis.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Zhi-Min Liu, Yu Yang, Hua Wang, Yan-Li Liu, Guo-Li Shen, Ru-Qin Yu,