Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10410990 | Sensors and Actuators B: Chemical | 2005 | 5 Pages |
Abstract
Enantioselective, potentiometric membrane electrodes (EPMEs) based on carbon paste are constructed. The carbon paste is modified with antibiotics: vancomycin, teicoplanin, and teicoplanin modified with acetonitrile. The proposed electrodes can be used reliably for enantiopurity tests of d-2-hydroxyglutaric acid using direct potentiometric method of analysis. The linear concentration ranges recorded for EPMEs are 10â7 to 10â3, 10â7 to 10â2 and 10â6 to 10â2Â mol/L with detection limits of 1.00Â ÃÂ 10â8, 1.00Â ÃÂ 10â8, 1.00Â ÃÂ 10â7Â mol/L for the vancomycin, teicoplanin and teicoplanin modified with acetonitrile based EPME, respectively. The selectivity was determined over l-2-hydroxyglutaric acid, creatine, creatinine and some inorganic cations. The proposed EPMEs were applied for the assay of d-2-hydroxyglutaric acid in urine samples. The duration of one analysis is 10Â min, including the calibration of the instrument and the determination of the amount of d-2-hydroxyglutaric acid in the urine sample.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Raluca-Ioana Stefan, R'afat Mahmoud Nejem, Jacobus Frederick van Staden, Hassan Y. Aboul-Enein,