| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10411073 | Sensors and Actuators B: Chemical | 2005 | 6 Pages |
Abstract
Anti-vitellogenin antibody was immobilized on a quartz crystal oscillator and applied to a quartz crystal microbalance (QCM) for detection of plasma vitellogenin (Vtg) of fish. The QCM exhibited a significant decrease in frequency responding to Vtg addition. The association constant K for the antigen-antibody reaction was determined to be 7 à 107 Mâ1. The QCM was also sensitive to plasma from Koi carp (Cyprinus carpio), of which Vtg synthesis had been stimulated by injection of estradiol-17β. Although nonspecific response to components in the plasma was observed, it was suppressed by pretreatment of the QCM device with bovine serum albumin.
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Authors
Kenji Oshima, Hiromichi Nakajima, Shouji Takahashi, Yoshio Kera, Masato Shimomura, Shinnosuke Miyauchi,