Biomicrosystem design and fabrication for the human papilloma virus 16 detection

In this paper, a biomicrosystem consisting of 98 biosensors based on the monoclonal antibody (mAb) 5051 immobilization for the detection of the human papilloma virus (HPV) 16 was designed and fabricated. The mAb 5051 immobilization was performed on a self-assembled monolayer of 4-aminothiophenol, on a polymethylmethacrylate substrate with a gold nanolayer. The biomicrosystem carries out 98 in situ different simultaneous tests without specialized equipment or personnel. The biomicrosystem characterization was performed by electrochemical impedance spectroscopy, cyclic voltammetry, impedance measurements, AFM and SEM. The biomicrosystem performance was verified by using positive and negative HPV 16 real samples from women, after 1 h incubation process using 40 biosensors from 4 different biomicrosystems. The HPV 16 samples were verified by a standard PCR test. An analytic hierarchy process was performed based on costs, effectiveness, time and operative characteristics between the biomicrosystem and traditional techniques for the HPV detection, such as PCR-RT and Hybrid Capture 2; concluding that the biomicrosystem is a dominant alternative with the 38.8% in the global score compared to the 34.5% of PCR-RT and the 26.7% of HC2.