Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10412760 | Sensors and Actuators B: Chemical | 2015 | 8 Pages |
Abstract
We have synthesized a highly pH and temperature-sensitive, fluorescent, crosslinked poly(N-isopropylacrylamide) (PNIPAM)-based probe via simple free radical emulsion copolymerization, which formed a reversible microgel containing both fluorophores of fluorescein and rhodamine derivatives. We confirmed that the opening of the lactone ring of fluorescein and the lactam ring of rhodamine in the polymer was responsible for the fluorescence changes in basic and acidic conditions, which showed fluorescence emissions at 514Â nm (green) and 586Â nm (red), respectively. The polymeric microgel exhibited a linear relationship between the ratio of emission intensity and pH windows of pH 2-6 and 7-12. Moreover, the microgel exhibited changes in fluorescence intensity with an alteration of turbidity of the microgel induced by temperature. Moreover, these fluorescence changes could be easily observed by the naked eye, serving as a potential probe for ratiometric microgel detection of pH or temperature.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yongkyun Kim, Daigeun Kim, Geunseok Jang, Jongho Kim, Taek Seung Lee,