Direct determination of kanamycin in raw materials, veterinary formulation and culture media using a novel liquid chromatography-evaporative light scattering method

In kanamycin acid sulfate pharmaceutical raw materials, the simultaneous determination of sulfate (tR = 2.1 min, LOD = 2.3 μg ml−1, %R.S.D. = 1.7, r > 0.9998) and kanamycins was feasible. No significant difference (t-test) was found between the results of the developed LC-ELSD method and those of reference methods, while recovery from kanamycin B spiked samples ranged from 95 to 105%. The developed method was also applied with very good accuracy for the determination of kanamycin A in veterinary formulation (%recovery 95-103, %R.S.D. < 1.4, n = 3) and for the determination of kanamycins A and B in bacteria culture media (%recovery 102 and 99, respectively).