Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10530058 | Analytica Chimica Acta | 2005 | 6 Pages |
Abstract
A fast HPLC method has been developed for simultaneous determination of rifabutin and its synthesis precursors. The analytes are separated in 1.8 min by means of a Kromasil 100 C18 column (50 mm Ã 2.1 mm i.d., 3.5 μm) at 30 °C. The mobile phase (A: 5 mM KH2PO4 adjusted to pH 6.5 with KOH; B: acetonitrile) was pumped at a flow rate of 0.4 ml minâ1 according to the fast gradient mode: 0 min, 58% B; 0-0.4 min, 95% B. Detection was by ultraviolet absorbance at 275 nm. The method was validated in accordance with the International Conference on Harmonisation (ICH) guidelines and good accuracy, intermediate precision (â¤4.6%) and linearity in the range 5-50 mg lâ1 were observed for all compounds. This method is sensitive (limits of detection ranged between 0.1 and 0.3 mg lâ1) and selective to quantify rifabutin and its synthesis precursors and could be used for in-process control.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Domingo Blanco Gomis, Noé Sánchez Núñez, Elena Andrés GarcÃa, Pilar Arias Abrodo, Miguel Bayod Jasanada, Mª. Dolores Gutiérrez Álvarez,