Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10566847 | Bioelectrochemistry | 2005 | 8 Pages |
Abstract
A novel chemically modified electrode based on the multiwall carbon nanotubes (MWNTs) film-coated carbon fiber ultramicroelectrode (CFUE) has been described for the determination of nitric oxide radical (·NO). The electrochemical behaviors of MWNTs-modified CFUE have been characterized in 0.2 mmol Lâ1 K4Fe(CN)6 and 0.1 mol Lâ1 KCl solution. The Nafion film was used to avoid some electroactive interferences. The amount of Nafion was optimized, and some possible interferents [such as nitrite (NO2â), nitrate (NO3â), ascorbate, dopamine (DA), l-arginine (l-Arg), etc.] were tested and evaluated. The oxidation peak current of ·NO increases significantly at the MWNT/Nafion-modified CFUE, in contrast to that at the bare and the Nafion-modified CFUE, and the oxidation peak potential is at 0.78 V (vs. SCE), which can be used for the detection of ·NO. The oxidation peak current is linearly with the concentration of ·NO from 2Ã10â7 to 8.6Ã10â5 mol Lâ1, and the detection limit is 2Ã10â8 mol Lâ1. The liver mitochondria in Carassius auratus were isolated and ·NO release from mitochondria was monitored by using this ultramicroelectrode system.
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Authors
Yazhen Wang, Qing Li, Shengshui Hu,