| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10706883 | Current Applied Physics | 2005 | 4 Pages |
Abstract
Next one is a genetical construction of postsynapse model cell for an evaluation of extracellular neural transmitter. Postsynaptic function is presented by channel gate receptor on postsynaptic membrane. In the present study, cloned glutamic acid channel gate receptor (GluR-D) gene is transformed into insect cell Sf-9. Sf-9 is easy to cultivate and is able to express GluR-D on its cell surface as same as postsynaptic membrane. Its function can be monitored by outer cell potential. Both of tactics is successfully performed and is applicatable cultured cell-based assay on pharmaceutical screening and chemical safety proofing.
Related Topics
Physical Sciences and Engineering
Physics and Astronomy
Condensed Matter Physics
Authors
Tetsuya Haruyama, Hitoshi Asakawa, Satoshi Migita, Shinya Ikeno,