Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10747270 | Acta Histochemica | 2012 | 9 Pages |
Abstract
Expression of ADAMs was analyzed using quantitative reverse-transcription polymerase chain reaction in laser-capture microdissected specimens of breast cancers and corresponding non-neoplastic breast tissues from 92 patients. The proteins' expression was confirmed by immunohistochemistry. Significantly elevated amounts of ADAM12L, ADAM12S and ADAM17 transcripts were found in malignant breast cells compared with normal breast tissue and both ADAMs proteins showed moderate to strong immunoexpression in tumor cells and peritumoral fibroblasts. ADAM12L and ADAM12S expressions were correlated with age, younger patients having higher expression of ADAM12L and ADAM12S; ductal cancers had higher expression of ADAM12L compared with lobular types, whereas ADAM12S was higher expressed in lobular cancers; higher expressions were found for both ADAM12 and ADAM17 in HER2/neu positive and highly proliferative cancers. High-grade cancers showed significantly increased expression of ADAM17. Our study on laser-capture microdissected specimens confers motivation for future work on development of ADAM-selective inhibitors for treatment of breast cancers.
Keywords
ΔΔCtQ-RT-PCRADAM12Tgf-αΔCTTACECLiHB-EGFADAM17EGFRGPiLCMDCISGAPDHTGF-βmRNAEGFIGFCDIDNAFFPES.D.ADAMdeoxyribonucleic acidstandard deviationIHCImmunohistochemistryHER2/neuGene expressionTumor grademessenger ribonucleic acidRINBreast cancersRNA Integrity Numberepidermal growth factortumor growth factor betaInsulin-like growth factorLaser-capture microdissectionformalin-fixed paraffin embeddedInvasive lobular carcinomaInvasive ductal carcinomaglyceraldehyde-3-phosphate dehydrogenaseEstrogen receptorHuman epidermal growth factor receptor 2Epidermal growth factor receptorProgesterone receptor
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Authors
Diana Narita, Edward Seclaman, Sorin Ursoniu, Andrei Anghel,