Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10748233 | Biochemical and Biophysical Research Communications | 2016 | 6 Pages |
Abstract
Nek11, a member of the never in mitosis gene A (NIMA) family, is activated in somatic cells associated with G1/S or G2/M arrest. However, its function in meiosis is unknown. In this research, the expression, localization and functions of NEK11 in the mouse oocyte meiotic maturation were examined. Western blotting indicated that NEK11S was the major NEK11 protein in mouse oocyte. MYC-tagged Nek11 mRNA microinjection and immunofluorescent staining showed that NEK11 was localized to the meiotic spindles at MI and MII stage. Knockdown of Nek11 by microinjection of siRNA did not affect germinal vesicle breakdown (GVBD) and the first polar body extrusion, but caused formation of 2-cell-like eggs. These results demonstrate that Nek11 regulates asymmetric cell division during oocyte meiotic maturation.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Lei Guo, Zhen-Bo Wang, Hong-Hui Wang, Teng Zhang, Shu-Tao Qi, Ying-Chun Ouyang, Yi Hou, Qing-Yuan Sun,