| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10748278 | Biochemical and Biophysical Research Communications | 2016 | 5 Pages |
Abstract
The CRISPR-Cas9 genome editing system has been widely used in multiple cells and organisms. Here we developed a CRISPR-Cas9 based in vitro large DNA vector editing system, using the Ad5-based vector as an example. We demonstrate use of this system to generate targeted mutations, in-frame gene deletion, and gene replacement. This in vitro CRISPR editing system exhibits high efficiency and accuracy. We believe this system can be applied in a variety of experimental settings.
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Authors
Lichun Tang, Mengmeng Gong, Pumin Zhang,