Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10748323 | Biochemical and Biophysical Research Communications | 2016 | 4 Pages |
Abstract
The generation of singlet oxygen during the oxidation of tyrosine and L-dopa using mushroom tyrosinase in a phosphate buffer (pH 7.4), the model of melanin synthesis in melanocytes, was examined. The reaction was performed in the presence of 2,2,6,6-tetramethyl-4-piperidone (4-oxo-TEMP), an acceptor of singlet oxygen and the electron spin resonance (ESR) of the spin adduct, 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy (4-oxo-TEMPO), was measured. An increase in the ESR signal attributable to 4-oxo-TEMPO was observed during the oxidation of tyrosine and L-dopa with tyrosinase, indicating the generation of singlet oxygen. The results suggest that 1O2 generation via tyrosinase-catalyzed melanin synthesis occurs in melanocyte.
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Authors
Akimitsu Miyaji, Masahiro Kohno, Yoshihiro Inoue, Toshihide Baba,