Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10748498 | Biochemical and Biophysical Research Communications | 2016 | 8 Pages |
Abstract
This study established a wheat transcriptome library using RH8706-49 and RH8706-34. Salt-induced differential genes were screened by Illumina RNA sequencing (RNA-Seq). Five differential genes were chosen to study the functions by combining transcript sequencing result and gene chip. The expression changes of these five differential genes were analyzed using real-time quantitative PCR (qRT-PCR) technique to determine the reliability and accuracy of transcriptome sequencing and transplanted into Arabidopsis thaliana to obtain transgenic homozygote plants for the salt tolerance test. The salt tolerance test results show that the transgenic plants grew far better than the wild-type plant.
Keywords
EMSKEGGEthyl Methyl Sulfonateprotein family databaseSelf-organizing mappingBWAKOGPfamRPKMKEGG Automatic Annotation ServerqRT-PCRqPCRFDRRNA-seqCDDSOMESTSalt toleranceTranscriptomeExpressed Sequence TagKAASKyoto Encyclopedia of Genes and Genomesfalse discovery rateGene ontologyquantitative polymerase chain reactionconserved domain databasenon-redundant databaseTriticum aestivum
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Authors
Xiaoli Ma, Peihan Gu, Wenji Liang, Yuxin Zhang, Xiaoli Jin, Shaoxiong Wang, Yinzhu Shen, Zhanjing Huang,