| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10748766 | Biochemical and Biophysical Research Communications | 2016 | 6 Pages |
Abstract
Group IVA phospholipase A2 (IVA-PLA2), which generates arachidonate, plays a role in inflammation. IVA-PLA2-deficiency reduced hepatotoxicity and hepatocyte cell death in mice that received a single dose of carbon tetrachloride (CCl4) without any inhibitory effects on CCl4-induced lipid peroxidation. An immunoblot analysis of extracts from wild-type mouse- and IVA-PLA2 KO mouse-derived primary hepatocytes that transiently expressed microtubule-associated protein 1 light chain 3B (LC3) revealed a higher amount of LC3-II, a typical index of autophagosome formation, in IVA-PLA2-deficient cells, suggesting the enhancement of constitutive autophagy. IVA-PLA2 may promote CCl4-induced cell death through the suppression of constitutive autophagy in hepatocytes.
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Authors
Keiichi Ishihara, Shiho Kanai, Kikuko Tanaka, Eri Kawashita, Satoshi Akiba,