Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10748822 | Biochemical and Biophysical Research Communications | 2016 | 8 Pages |
Abstract
Hypoxia promotes cancer progression, and metastasis. The major protein expressed in hypoxic solid cancer is hypoxia-inducible factor 1 (HIF1). We show that enhanced phosphorylation of a conventional protein kinase C isoform, PKCα, at threonine 638 (T638) by hypoxia-mimetic cobalt chloride induces HIF1α in nuclei of gastric epithelial cells (GECs). Moreover, phospho-T638-PKCα (P-PKCα) interacts with p300-HIF1α complex in the nuclei of hypoxic GECs and PKCα phosphorylation at T638 enhances transcriptional activity of HIF1α. High P-PKCα expression in neoplastic gastric cancer biopsy samples as compared to nonneoplastic samples suggests that P-PKCα might act as an indicator of gastric cancer progression.
Keywords
PVDFGECsHIF1αHREHDAC1NLSPKCHIF1P300DAPIsodium dodecyl sulphate-polyacrylamide gel electrophoresisSDS-PAGEPhDsdiacylglycerolDAGGastric epithelial cellGastric epithelial cellsnuclear localization signalhypoxia-inducible factor 1Hypoxia-response elementdominant negativewild typeHypoxiaProtein kinase Cprolyl hydroxylasesPolyvinylidene fluorideCobalt chloride
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Authors
Suvasmita Rath, Aditya Anand, Nilabh Ghosh, Lopamudra Das, Shrikant B. Kokate, Pragyesh Dixit, Swetapadma Majhi, Niranjan Rout, Shivaram P. Singh, Asima Bhattacharyya,