Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10749356 | Biochemical and Biophysical Research Communications | 2016 | 10 Pages |
Abstract
This study was designed to evaluate whether CSE/H2S system, which is regulated by miR-216a, regulated ABCA1-mediated cholesterol efflux and cholesterol contents in THP-1 macrophages-derived foam cells. Our qPCR and western blotting results showed that CSE/H2S significantly up-regulated the expression of ATP-binding cassette transporter A1 (ABCA1) mRNA and protein via PI3K/AKT pathway in foam cells derived from human THP-1 macrophages. The miR-216a directly targeted 3â² untranslated region of CSE. It significantly reduced CSE and ABCA1 expression, and also decreased the phosphorylation of PI3K and AKT. Additionally, cholesterol efflux decreased, and cholesterol levels increased in THP-1 macrophage-derived foam cells in response to treatment with miR-216a. Our study demonstrates that CSE/H2S system is regulated by miR-216a, and regulates ABCA1-mediated cholesterol efflux and cholesterol levels through the PI3K/AKT pathway.
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Authors
Duo Gong, Hai-peng Cheng, Wei Xie, Min Zhang, Dan Liu, Gang Lan, Chong Huang, Zhen-wang Zhao, Ling-yan Chen, Feng Yao, Yu-lin Tan, Liang Li, Xiao-dan Xia, Xi-long Zheng, Zong-bao Wang, Chao-ke Tang,