| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10750138 | Biochemical and Biophysical Research Communications | 2015 | 7 Pages |
Abstract
In this study, we report the characterization of biological and structural properties of dioicin 1, including the determination of its primary structure by using a combined approach based on Edman degradation, de novo sequencing by ESI-Q-TOF-MS/MS and peptide mapping by MALDI-TOF MS. Knowledge of dioicin 1 primary structure provide us a mean to deepen Phytolaccaceae's RIPs phylogeny. We speculate that both dioicins 1 and 2 share common ancestors with PAP-II and PAP icos-II and that dioicin 1 is not closely related to other members of this clade, thus shedding lights on evolutionary relationships among type 1 RIPs from Phytolaccaceae.
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Authors
Rosita Russo, Angela Chambery, Valeria Severino, Augusto Parente, Antimo Di Maro,