Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10750616 | Biochemical and Biophysical Research Communications | 2015 | 6 Pages |
Abstract
MAPKKK is the largest family of MAPK cascade, which is known to play important roles in plant growth, development and immune responses. So far, only a few have been functionally characterized even in the model plant, Arabidopsis due to the potential functional redundancy of MAPKKK. We previously identified and cloned a few MAPKKK family genes from rapeseed. In this study, BnaMAPKKK4 was characterized as a member in eliciting accumulation of reactive oxygen species (ROS) and hypersensitive response (HR)-like cell death. This is accompanied with accumulation of malondialdehyde (MDA), anthocyanin as well as nuclear DNA fragmentation. The transcript abundance of a series of ROS accumulation, cell death, and defense response related genes were up-regulated by the expression of MAPKKK4. Further investigation identified BnaMAPKKK4 elicited ROS through the downstream MPK3. These results indicate that BnaMAPKKK4 and its downstream components function in the ROS-induced cell death.
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Biochemistry
Authors
Liang Li, Chaofei Ye, Rui Zhao, Xin Li, Wu-zhen Liu, Feifei Wu, Jingli Yan, Yuan-Qing Jiang, Bo Yang,