Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10751183 | Biochemical and Biophysical Research Communications | 2015 | 7 Pages |
Abstract
Although autophagy regulates the quality and quantity of cellular organelles, the regulatory mechanisms of peroxisomal autophagy remain largely unknown. In this study, we developed a cell-based image screening assay, and identified 1,10-phenanthroline (Phen) as a novel pexophagy inducer from chemical library screening. Treatment with Phen induces selective loss of peroxisomes but not endoplasmic reticulum and Golgi apparatus in hepatocytes. In addition, Phen increases autophagic engulfment of peroxisomes in an ATG5 dependent manner. Interestingly, treatment of Phen excessively produces peroxisomal reactive oxygen species (ROS), and inhibition of the ROS suppresses loss of peroxisome in Phen-treated cells. Taken together, these results suggest that Phen triggers pexophagy by enhancing peroxisomal ROS.
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Authors
Doo Sin Jo, Dong-Jun Bae, So Jung Park, Hae Mi Seo, Han Byeol Kim, Jeong Su Oh, Jong Wook Chang, Sang-Yeob Kim, Jung-Won Shin, Dong-Hyung Cho,