Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10751262 | Biochemical and Biophysical Research Communications | 2015 | 7 Pages |
Abstract
Alteration in specific inertial conditions can lead to changes in morphology, proliferation, mechanical properties and cytoskeleton of cells. In this report, the effects of hypergravity on morphology of Adipose-Derived Stem Cells (ADSCs) are indicated. ADSCs were repeatedly exposed to discontinuous hypergravity conditions of 10Â g, 20Â g, 40Â g and 60Â g by utilizing centrifuge (three times of 20Â min exposure, with an interval of 40Â min at 1Â g). Cell morphology in terms of length, width and cell elongation index and cytoskeleton of actin filaments and microtubules were analyzed by image processing. Consistent changes observed in cell elongation index as morphological change. Moreover, cell proliferation was assessed and mechanical properties of cells in case of elastic modulus of cells were evaluated by Atomic Force Microscopy. Increase in proliferation and decrease in elastic modulus of cells are further results of this study. Staining ADSC was done to show changes in cytoskeleton of the cells associated to hypergravity condition specifically in microfilament and microtubule components. After exposing to hypergravity, significant changes were observed in microfilaments and microtubule density as components of cytoskeleton. It was concluded that there could be a relationship between changes in morphology and MFs as the main component of the cells.
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Authors
Alireza Tavakolinejad, Mohsen Rabbani, Mohsen Janmaleki,