Article ID Journal Published Year Pages File Type
10751938 Biochemical and Biophysical Research Communications 2015 7 Pages PDF
Abstract
Fusion to a SUMO moiety can substantially increase soluble expression levels of certain hard to express terpene synthases in E. coli. The kinetic data determined for the recombinant synthase are comparable to other described plant sesquiterpene synthases and in the typical range of enzymes belonging to the secondary metabolism. This leaves potential for optimizing catalytic parameters through methods like directed evolution.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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