Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10751979 | Biochemical and Biophysical Research Communications | 2015 | 7 Pages |
Abstract
MVs are released in response to several stress agents, in an attempt to prevent continued cellular damage. After an initial stimulus of prostate cancer cells with sublytic C5b-9 and activation of MV release through PKC, cells take at least 20 min to fully recover their ability to microvesiculate. This release of MVs through activation of sublytic C5b-9 was inhibited by the PKC inhibitor bisindoylmaleimide I but not the Rho kinase inhibitor, Y27632. After stimulus there is a rise of 79 nMsâ1 over 11 s, reaching a peak [Ca2+]i of 920 nM. The concentration of cytosolic calcium then falls steadily at 2.4 nMsâ1 over 109 s reaching baseline levels (50-100 nM) within 10-15 min. In PC3 cells the rate of release of MVs from stimulated cells also reaches a minimum within 10-15 min. Using fura-2 AM-loaded cells, upon stimulation, cells were found to release MVs with a concentration of intravesicular calcium estimated at â¼430 nM.
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Authors
Dan Stratton, Colin Moore, Lei Zheng, Sigrun Lange, Jameel Inal,