Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10753307 | Biochemical and Biophysical Research Communications | 2015 | 6 Pages |
Abstract
Fatty acid biosynthesis type II in mycobacteria delivers the fatty acids required for mycolic acid synthesis. The pathway employs a unique maoC like β-hydroxyacyl-ACP dehydratase HadAB or HadBC heterodimer in the third step of the elongation cycle. Here we report the crystal structure of the HadAB complex determined using a Pb-SIRAS method. Crystal structure aided with enzymatic study establishes the roles of HadA as a scaffolding component and HadB as a catalytic component together indispensable for the activity. The detailed structural analysis of HadAB in combination with MD simulation endorses the spatial orientation of the central hot-dog helix and the dynamic nature of its associated loop in regulation of substrate specificities in dehydratase/hydratase family enzymes.
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Authors
Rupam Biswas, Anirudha Dutta, Debajyoti Dutta, Ditipriya Hazra, Deb Ranjan Banerjee, Amit Basak, Amit Kumar Das,