Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10753555 | Biochemical and Biophysical Research Communications | 2015 | 6 Pages |
Abstract
Previous studies demonstrated that depletion of membrane cholesterol by 10Â mM methyl-beta-cyclodextrin (MCD) results in increased spontaneous exocytosis at both peripheral and central synapses. Here, we investigated the role of protein kinase C in the enhancement of spontaneous exocytosis at frog motor nerve terminals after cholesterol depletion using electrophysiological and optical methods. Inhibition of the protein kinase C by myristoylated peptide and chelerythrine chloride prevented MCD-induced increases in FM1-43 unloading, whereas the frequency of spontaneous postsynaptic events remained enhanced. The increase in FM1-43 unloading still could be observed if sulforhodamine 101 (the water soluble FM1-43 quencher that can pass through the fusion pore) was added to the extracellular solution. This suggests a possibility that exocytosis of synaptic vesicles under these conditions could occur through the kiss-and-run mechanism with the formation of a transient fusion pore. Inhibition of phospholipase C did not lead to similar change in MCD-induced exocytosis.
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Authors
Alexey M. Petrov, Guzalija F. Zakyrjanova, Anastasia A. Yakovleva, Andrei L. Zefirov,