Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10753784 | Biochemical and Biophysical Research Communications | 2014 | 6 Pages |
Abstract
We proposed to use a novel stepwise sequence-constructive SELEX method to develop DNA aptamers that can recognize Globo H which is a tumor-associated carbohydrate antigen. A combinatorial synthetic library that consisted of DNA molecules with randomized regions of 15-bases was used as the starting library for the first SELEX procedure. The input DNA library for the second round of SELEX consisted of the extension of the 5â² and 3â²-ends with 7-bases that were randomized from four selected aptamers. The third round of SELEX was performed following the same procedures as described for the second round of SELEX. The experimental results indicate that the binding affinity of DNA aptamers to Globo H was enhanced when using the sequence-constructive SELEX approach. The selectivity of the DNA aptamers for related disaccharides, mannose derivatives, and Globo H analogs demonstrated the ability of the DNA aptamers to discriminate the presence of various glycans with different structures.
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Authors
Chin-Yu Wang, Chung-Yi Wu, Ting-Chun Hung, Chi-Huey Wong, Chung-Hsuan Chen,