Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10753792 | Biochemical and Biophysical Research Communications | 2014 | 6 Pages |
Abstract
Symbiotic protists in the gut of termites are prominent natural resources for enzymes involved in lignocellulose degradation. Here we report expression, purification, and biochemical characterization of a glycoside hydrolase family 26 mannanase RsMan26H from the symbiotic protist of the lower termite, Reticulitermes speratus. Biochemical analysis of RsMan26H demonstrates that this enzyme is an endo-processive mannobiohydrolase producing mannobiose from oligo- and polysaccharides, followed by a minor accumulation of oligosaccharides larger than mannobiose. To our knowledge, this is the first report describing the unique mannobiohydrolase enzyme from the eukaryotic origin.
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Authors
Hikaru Tsukagoshi, Akihiko Nakamura, Takuya Ishida, Masato Otagiri, Shigeharu Moriya, Masahiro Samejima, Kiyohiko Igarashi, Katsuhiko Kitamoto, Manabu Arioka,