Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10753983 | Biochemical and Biophysical Research Communications | 2014 | 34 Pages |
Abstract
We used a human ameloblastoma cell line (AM-3 ameloblastoma cells), human fibroblasts (HFF-2 fibroblasts), and primary-cultured fibroblasts from human ameloblastoma tissues, and analyzed the effect of ameloblastoma-associated cell-to-cell communications on gene expression, cytokine secretion, cellular motility and proliferation. AM-3 ameloblastoma cells secreted higher levels of interleukin (IL)-1α than HFF-2 fibroblasts. Treatment with conditioned medium from AM-3 ameloblastoma cells upregulated gene expression and secretion of IL-6 and IL-8 of HFF-2 fibroblasts and primary-cultured fibroblast cells from ameloblastoma tissues. The AM3-stimulated production of IL-6 and IL-8 in fibroblasts was neutralized by pretreatment of AM-3 cells with anti-IL-1α antibody and IL-1 receptor antagonist. Reciprocally, cellular motility of AM-3 ameloblastoma cells was stimulated by HFF-2 fibroblasts in IL-6 and IL-8 dependent manner. In conclusion, ameloblastoma cells and stromal fibroblasts behave interactively via these cytokines to create a microenvironment that leads to the extension of ameloblastomas.
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Authors
Takao Fuchigami, Toshiro Kibe, Hirofumi Koyama, Shosei Kishida, Mikio Iijima, Yoshiaki Nishizawa, Hiroshi Hijioka, Tomomi Fujii, Masahiro Ueda, Norifumi Nakamura, Tohru Kiyono, Michiko Kishida,