| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10754639 | Biochemical and Biophysical Research Communications | 2014 | 7 Pages |
Abstract
MoxXT module of Bacillus anthracis encodes MoxX, a labile protein and MoxT, a ribonuclease. However, mechanism of cleavage of RNA by MoxT has not been explored till date. In the present study, we have demonstrated that MoxT is a sequence specific ribonuclease which recognizes UACAU sequence in ss RNA and cleaves between U and A. Moreover, cleavage of RNA requires 2â² OH group of first residue, i.e. U of UACAU RNA sequence. An interesting finding which makes it distinct from the other MazF family toxins was also observed, i.e. its ability to cleave RNA in DNA-RNA hybrid.
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Authors
Shashikala Verma, Rakesh Bhatnagar,