Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10754912 | Biochemical and Biophysical Research Communications | 2014 | 23 Pages |
Abstract
We identified a novel elongase gene from a selected strain of the Oomycete, Pythium sp. BCC53698. Using a PCR approach, the cloned gene (PyElo) possessed an open reading frame (ORF) of 834 bp encoding 277 amino acid residues. A similarity search showed that it had homology with the PUFA elongases of several organisms. In addition, the signature characteristics, including four conserved motifs, a histidine-rich catalytic motif and membrane-associated feature were present in the Pythium gene. Heterologous expression in Saccharomyces cerevisiae showed that it was specific for fatty acid substrates, having a double bond at Î6-position, which included γ-linolenic acid (GLA) and stearidonic acid (STA), and preferentially elongated the n3-18C PUFA. This is an elongase in Oomycete fungi, which displays very high specificity on Î6-18C desaturated fatty acids. This will be a powerful tool to engineer PUFA biosynthesis in organisms of interest through the n-6 series pathway for producing value-added fatty acids.
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Authors
Sukanya Jeennor, Pattsarun Cheawchanlertfa, Sarinya Suttiwattanakul, Sarocha Panchanawaporn, Chanikul Chutrakul, Kobkul Laoteng,