Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10755019 | Biochemical and Biophysical Research Communications | 2014 | 6 Pages |
Abstract
In the present study, we demonstrated the reciprocal regulation of hypoxia-inducible factor 1 alpha (HIF1A) gene expression via untranslated region-(UTR) dependent mechanisms. A 151 nucleotide sequence found in the HIF1A 5â²-UTR is sufficient for significant translational up-regulation. On the other hand, the 3â²-UTR of HIF1A has been implicated in mRNA degradation. In the non-metastatic breast cancer cell line MCF7, the 3â²-UTR-dependent down-regulatory machinery predominates over the 5â²-UTR-dependent up-regulation of HIF1A. However, 5â²-UTR-dependent up-regulation is dominant among metastatic cell lines (MDA-MB453, U87MG). It is therefore likely that the predominance of 5â²-UTR-dependent translational enhancement of HIF1A is critical for the malignant phenotype of cancer cells. PTBP-1, but not HuR, is a candidate RNA binding protein for the translational control of HIF1A.
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Authors
Motoaki Yasuda, Tomoyuki Hatanaka, Hiroki Shirato, Takeshi Nishioka,