Structure and flexibility of the tropomyosin overlap junction

- Head-to-tail linkage is required for tropomyosin cable formation on F-actin.
- Molecular dynamics of tropomyosin overlap domains were carried out.
- N- to C-terminal coiled-coils face each other at an orthogonal angle.
- The bending angle of the overlap domain and the rest of the molecule are comparable.
- This curvature ensures tropomyosin assembly as an uninterrupted cable on F-actin.