Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10755513 | Biochemical and Biophysical Research Communications | 2014 | 6 Pages |
Abstract
To explore the estrogen-regulated genes genome-widely in breast cancer, cap analysis of gene expression (CAGE) sequencing was performed in MCF-7 cells under estrogen treatment. Estrogen-regulated expressional changes were found in 1537 CAGE tag clusters (TCs) (⩾1.5 or ⩽0.66-folds). Among them, 15 TCs were situated in the vicinity of (⩽10 kb) reported estrogen receptor-binding sites. Knockdown experiments of the 15 TC-associated genes demonstrated that the genes such as RAMP3, ISOC1 and GPRC5C potentially regulate the growth or migration of MCF-7 cells. These results suggest that CAGE sequencing will reveal novel estrogen target genes in breast cancer.
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Authors
Ryonosuke Yamaga, Kazuhiro Ikeda, Joost Boele, Kuniko Horie-Inoue, Ken-ichi Takayama, Tomohiko Urano, Kaoru Kaida, Piero Carninci, Jun Kawai, Yoshihide Hayashizaki, Yasuyoshi Ouchi, Michiel de Hoon, Satoshi Inoue,